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Channel Steps
1 2 3 4 5 6
1 0.4M EDC/ 0.1M NHS
Activation
1X PBS
Washing
Anti-CRP (0.1mg/ml)
10mM Sodium acetate pH5
1X PBS
Washing
1M Ethanolamine
Blocking
1X PBS
Washing
2 0.4M EDC/ 0.1M NHS
Activation
1X PBS
Washing
Anti-CRP (0.1mg/ml)
1X PBS pH7.4
1X PBS
Washing
1M Ethanolamine
Blocking
1X PBS
Washing
3 0.4M EDC/ 0.1M NHS
Activation
1X PBS
Washing
IgG (1mg/ml)
1X PBS pH7.4
1X PBS
Washing
1M Ethanolamine
Blocking
1X PBS
Washing